Fig. 2.

Mice carrying a LRRK2 mutation (D1994S) that silences kinase activity (kinase-dead; D1994S KD) showed similar motor phenotype of wild-type littermates (WT). Motor performance was evaluated in two cohorts of mice following a longitudinal phenotyping approach. Mice were subjected to a set of tests, namely the bar (A), drag (B) and rotarod (C) tests, starting at 3 months up to 19 months of age. In addition, an open field test was performed in 15-month-old animals (D–E). Motor activity was expressed as immobility time (sec; A, D), number of steps (B), time on rod (sec; C), and total distance traveled (m; E). Data are means ± SEM of 10 mice per group, and were analyzed using one-way RM ANOVA followed by the Newman–Keuls test for multiple comparisons. Differences between genotypes at the single time-point levels were evaluated using the Student t-test, two-tailed for unpaired data.

**P < 0.01 different from 3-month-old mice of the same genotype.