Fig. 6.

The LRRK2 kinase inhibitor H-1152 attenuated endogenous LRRK2 phosphorylation at Ser935 in striatum but not cerebral cortex of G2019S knock-in (G2019S KI) mice, being ineffective in wild-type littermates (WT). Three 12-month-old LRRK2 G2019S KI mice (A–B) were used as untreated controls, and three G2019S KI mice were administered with H-1152 (1 mg/kg, i.p.). In parallel, five WT KI mice (C–D) were used as controls, and four WT KI mice were treated with the same dose of H-1152 (1 mg/kg, i.p.). LRRK2 phosphorylation was measured ex-vivo in the striatum (A, C) and cerebral cortex (B, D), before (time 0; T0) and 20 min after H-1152 administration. Results are mean ± SEM of 3–5 mice per group, and were analyzed using the Student t-test, two tailed for unpaired data. To minimize experimental variability, each sample was loaded in duplicate and the numbers plotted represent the mean of the two technical replicates.

*P < 0.05 different from basal values (T0).