Fig. 9.

The LRRK2 kinase inhibitor Nov-LRRK2-11 attenuated endogenous LRRK2 phosphorylation at Ser935 in the striatum and cortex of both G2019S knock-in (G2019S KI) mice and wild-type littermates (WT). Three 12-month-old G2019S KI mice (A–B) were used as untreated controls, and three G2019S KI mice were administered with Nov-LRRK2-11 (10 mg/kg, i.p.). In parallel, five WT KI mice (C–D) were used as controls, and five WT KI mice were treated with the same dose of Nov-LRRK2-11 (10 mg/kg, i.p.). LRRK2 phosphorylation was measured ex vivo in the striatum (A, C) and cerebral cortex (B, D), before (time 0; T0) and 30 min after Nov-LRRK2-11 administration. Results are mean ± SEM of 3–5 mice per group, and were analyzed using the Student t-test, two tailed for unpaired data.

*P < 0.05, **P < 0.01 different from basal values (T0).