Skip to main content
. 2014 Oct 2;10:31. doi: 10.1186/1746-4811-10-31

Figure 10.

Figure 10

Near neutral leaf apoplast in Avena sativa requires Pt -GFP for detecting alkalizing effects. Leaf apoplastic pH response of Avena sativa as provoked by the addition of 25 mM Cl via L-cysteinium chloride into the nutrient solution. Time point of chloride addition as indicated by the arrow. pH, as quantified at the adaxial leaf face is plotted over time. Pt-GFP, grey curve; Oregon Green, black curve. Plants were cultivated with 15 mM nitrate in the nutrient solution given as Ca(NO3)2. Leaf apoplastic pH was averaged (n = 6 ROIs per ratio image and time point; mean ± SE of ROIs). Representative kinetics of eight equivalent recordings of plants gained from independent experiments (n = biological replicates).