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. 2014 Oct 2;10:31. doi: 10.1186/1746-4811-10-31

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© Geilfus et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vivo calibration of Pt -GFP fluorescence ratio (F ₄₉₀ /F ₃₈₇ ). The Boltzmann fit was chosen for fitting sigmoidal curves to calibration ratio data. Fitting resulted in an optimal dynamic range for pH measurements between 5.3 and 8.4. In vivo calibration was conducted on six different plants, each biological replicate was technically replicated. Data are mean of n = 6 ± SE.