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. 2014 Oct 2;10:31. doi: 10.1186/1746-4811-10-31

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© Geilfus et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nitrate nutrition alkalized the leaf apoplast into Pt -GFP’s range of responsiveness. Comparison between the responsiveness of Pt-GFP (grey) and Oregon Green (black) to apoplastic pH changes as induced by the addition of 25 mM Cl⁻ via L-cysteinium chloride to the roots of Vicia faba. Time point of chloride addition is indicated by the arrow. Plants were cultivated with 15 mM nitrate in the nutrient solution given as Ca(NO₃)₂. pH as quantified at the adaxial face of Vicia faba leaves is plotted over time. Leaf apoplastic pH was averaged (n = 6 ROIs per ratio image and time point; mean ± SE of ROIs). Representative kinetics of six equivalent recordings of plants gained from independent experiments (n = 6 biological replicates).