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. 2014 Sep 30;10:221. doi: 10.1186/s12917-014-0221-8

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© Vlasakova et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Prevalence of viruses in individual groups of pigs. Viruses were detected in pooled tissue samples (lymph nodes, liver and spleen) of diseased pigs of different age and lymph nodes of healthy animals. Total DNA or RNA was isolated by Chelex resins or TRIzol reagent, respectively. RNA was transcribed into cDNA using random hexamers (PTV) or gene-specific primer (PRRSV) and SuperScript III reverse transcriptase. Viral genomes were detected by PCR, RT-PCR or real-time PCR based on SYBR Green (TTSuV1 and TTSuV2).