Figure 4.
Cellular uptake of free daunomycin and daunomycin-conjugated TFO. (A) MCF-7 cells were incubated with 0.2 and 1 µM of daunomycin or dauno-GT11. After 4 h, cells were washed, harvested and collected by centrifugation. Uptake was then determined by flow cytometry. Untreated cells (control) were used to determine background fluorescence levels. (B) MCF-7 cells were incubated with 1 µM of daunomycin (a and b) or dauno-GT11 (c and d) for 6 h and then examined by fluorescence (top) and phase-contrast (bottom) microscopy.