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hFBH1 and SCFhFBH1 unwind longer duplex DNAs with similar efficiency. Three different partial duplex DNA substrates (30, 52 and 91 bp) were prepared as described in Materials and Methods. Reaction mixtures containing 15 fmol of each substrate and 110 fmol of either hFBH1 (A) or SCFhFBH1 (B) were incubated for the period of time indicated in the figure.
