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. 2014 Sep 12;58(3):2408. doi: 10.4081/ejh.2014.2408

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© Copyright R.J. Bułdak et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Viability and apoptosis ratio of human colorectal HCT-116 cells after cytochalasin-B treatment. a) Cells treated with 0, 1, 3 to 5g/mL cytochalasin-B for 24 h, cells viability quantified by MTS assay; results were obtained from seven experiments, bars represent average relative survival rate (%) ±SD compared to control; *P<0.05 and **P<0.001 indicate comparison between untreated samples and cytochalasin B-treated samples; percent of apoptotic cells in the groups treated with CytB solely or in combination with N-acetylcysteine (NAC; 5 mM). b) Cells observed under fluorescent microscope following nuclei staining with Hoechst 33258; bars represent mean ±SD of the percentage of cytokinesis failed HCT-116 cells that underwent apoptosis; **P<0.001 vs control.