Table 1.

Cell compartment area and the amount of visfatin antigen per 100 µm² surface area of HCT-116 cells in cytB-treated cells (3 and 5, g/mL) and untreated cultures. Data represent mean cell’s surface area presented in, µm² ± SD and mean amount of immunogold visfatin-bounded particles per 100 µm² surface area (cytosol, nucleus) in tested cells. Cytoplasm area was obtained from the subtraction of nuclear area from total cell area.

	Cell surface area (µm2)	Cell nucleus surface area (µm2)	Cell cytosol surface area (µm2)	Amount of visfatin antigen in nucleus/(µm2)	Amount of visfatin antigen in cytosol/100 µm2	Total amount of visfatin antigen/100 µm2
Control MN cells	57.8± 8.1	27±2.1	30.7±10.9	1470±326	2340±523	3810±340
Cytochalasin B (3 µg/mL) BN cells	132.2±10.4*	53.9±4.2*	78.2±9.0*	810±101*	640±98*	1460±99*
Cytochalasin B (5 µg/mL) BN cells	173.9± 12.3*	67.6±5.1*	106.3±11.8*	101±33*	74±12*	181±14*

MN, mono-nucleated (2n) diploid cells; BN, bi-nucleated (4n) tetraploid cells.

*P<0.05 vs control group (n=100 counted cells in five separate experiments).