Table 1.

Sequences of primers used in the Real-Time reverse transcription-polymerase chain reaction.

Genes	Forward primer	Reverse primer	Accession number	Product length (bp)
Aggrecan	ACACCCCTACCCTTGCTTCT	AAAGTGTCCAAGGCATCCAC	NM_022190.1	124
Type II collagen	CCTGAAACTCTGCCACCCAG	GTTCTTCCGAGGCACAGTCG	NM_012929.1	151
SOX-9	TACTTCCAGTGGAATAGAAG	TGCGCCCACACCATGA	XM_001081628.2	69
Type X collagen	GAAACAGGTGTCTGACTTAC	TACTTCCAGTGGAATAGAAG	XM_001053056.3	141
Type I collagen	CAGGCTGGTGTGATGGGATT	CCAAGGTCTCCAGGAACACC	NM_053304.1	278
Osteocalcin	GCCCTGACTGCATTCTGCCTCT	TCACCACCTTACTGCCCTCCTG	NM_013414.1	103
Ank	CAAGAGAGACAGGGCCAAAG	AAGGCAGCGAGATACAGGAA	NM_053714.1	177
GAPDH	CTCAACTACATGGTCTACATGTTCCA	CTTCCCATTCTCAGCCTTGACT	NM_017008.3	81