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. 2014 Sep 9;58(3):2422. doi: 10.4081/ejh.2014.2422

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© Copyright Y. Liu et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Western blot of of PPAR 2, leptin and leptin receptor in the oviduct tissues of Rana dybowskii, during breeding period (B) and pre-hibernation (P). Positive bands of PPAR 2, leptin and leptin receptor were observed in the position of about 52kDa (A), 16kDa (B) and 132kDa (C) respectively. ß-actin was used as controls to correct for loading in each lane. PC, positive control; NC, negative control. The expression levels were determined by densitometric analysis. Bars represent means ±SD for three independent experiments; asterisks indicate significant difference (P<0.01).