Figure 8. Teneurins function to regulate acetylcholine receptor number.
(A–C) Representative high magnification confocal z-stack images of DA1 and VA1d PNs expressing Dα7-GFP in control animals (A), ten-a mutants (B), or animals expressing RNAi against ten-m in the same PNs (C), and stained with antibodies against GFP (green) and N-Cadherin (blue). DA1 and VA1d glomeruli are outlined together (white dashed line) and were determined using the accompanying mtdT label (not shown). Due to partner matching defects in the ten-a mutant that prevent clear delineation between the two glomeruli, the combined count was compared across genotypes. (D) Quantification of Dα7-GFP puncta in the noted genotypes. (E) Quantification of PN neurite volume in all genotypes. (F) Quantification of AChR density in all genotypes. All observed phenotypes are statistically indistinguishable. Significance was assessed with a one-way ANOVA and corrected for multiple comparisons by a posthoc Tukey's multiple comparisons test. ***p < 0.001 in comparison with control. n.s. = not significant. In all cases, data represent mean ± SEM and n ≥ 8 animals, 16 antennal lobes. Scale bar = 5 μm.