Table 1. Proteins recovered from a global two-hybrid screen that interact with residues 1–975 of Sen1p.
| Protein | Length (amino acids) | No. times recovered | Residues in clone | Function | Cellular location |
|---|---|---|---|---|---|
| Gcr2p |
534 |
2 |
163–534 |
Transcription activator |
Nucleus |
| Nup49p |
472 |
1 |
21–472 |
Nuclear export |
Nuclear pore |
| Pyc2p |
1180 |
1 |
696–979 |
Pyruvate carboxylase |
Cytoplasm |
| Rad2p |
1031 |
3 |
327–672 |
DNA repair |
Nucleus |
| Rad2p |
|
1 |
327–544 |
|
|
| Rad2p |
|
1 |
491–606 |
|
|
| Rpo21p(Rpb1p) |
1733 |
3 |
1497–1733 |
RNA polymerase |
Nucleus |
| YPR148C |
435 |
2 |
95–435 |
Function unknown |
Cytoplasm |
| Prs3p |
320 |
1 |
142–204 |
|
Cytoplasm |
| Prs3p | 3 | 142–320 | Ribose-phosphate pyrophosphokinase 3 |
Information on the genes and proteins can be found in the Saccharomyces Genome Database (http://www.yeastgenome.org/).