Table 2.
ORF analysis of porothramycin biosynthetic gene cluster
| Gene | No. of amino acids | Highest GenBank Homology (BlastX) | % of Identity/Similarity | Proposed Function | Anthramycin gene cluster homology (BlastX) | % of Identity/ Similarity |
|---|---|---|---|---|---|---|
| por1 | 177 | YP_003114518 | 66/80 | NADPH-dependent FMN reductase | ||
| por2 | 227 | AFU02188 | 68/77 | two component transcriptional regulator | ORF25 (ABW71856) | 66/77 |
| por3 | 743 | WP_020540464 | 75/86 | ABC transporter (UvrA family) | ||
| por4 | 316 | WP_019357233 | 75/83 | DeoR family transcription regulator | ||
| por5 | 479 | ABW71855 | 70/81 | flavin-containing oxidoreductase | ORF24 (ABW71855) | 70/81 |
| por6 | 400 | ABW71841 | 70/82 | MFS transporter | ORF10 (ABW71841) | 70/82 |
| por7 | 258 | ABW71840 | 61/72 | hydroxylase/glyoxylase (bleomycin res) | ORF9 (ABW71840) | 61/72 |
| por8 | 622 | ABW71832 | 72/81 | amidotransferase | ORF1 (ABW71832) | 72/81 |
| por9 | 397 | ABW71835 | 73/83 | cytochrome P450 hydroxylase | ORF4 (ABW71835) | 73/83 |
| por10 | 350 | ABW71836 | 76/86 | C-Methyltransferase | ORF5 (ABW71836) | 76/86 |
| por11 | 599 | ABW71837 | 81/87 | gamma-glutamyltransferase | ORF6 (ABW71837) | 81/87 |
| por12 | 471 | ABW71838 | 73/82 | FAD oxidoreductase | ORF7 (ABW71838) | 73/82 |
| por13 | 156 | ABW71843 | 72/83 | L-DOPA 2,3-dioxygenase | ORF12 (ABW71843) | 72/83 |
| por14 | 305 | ABW71844 | 61/70 | L-Tyrosine 3-hydroxylase | ORF13 (ABW71844) | 61/70 |
| por15 | 297 | ABW71845 | 82/88 | F420 dependent reductase | ORF14 (ABW71845) | 82/88 |
| por16 | 289 | ABW71846 | 65/71 | unknown (putative isomerase) | ORF15 (ABW71846) | 65/71 |
| por17 | 415 | ABW71847 | 75/81 | kynureninase | ORF16 (ABW71847) | 75/81 |
| por18 | 347 | YP_006809167 | 73/85 | aromatic C-methyltransferase | ORF19 (ABW71850) | 73/81 |
| por19 | 288 | WP_017545377 | 71/76 | aryl formamidase | ORF20 (ABW71851) | 74/80 |
| por20 | 607 | ABW71852 | 73/84 | NRPS | ORF21 (ABW71852) | 73/84 |
| por21 | 1938 | ABW71853 + ABW71854 | 71/80 + 72/81 | NRPS + kynurenine 3-monooxygenase | ORF22 (ABW71853) + ORF23 (ABW71854) | 71/80 + 72/81 |
| por22 | 290 | WP_017974834 | 67/77 | NmrA family transcriptional regulator | ||
| por23 | 477 | WP_005249919 | 76/86 | FMNH2-dependent monooxygenase | ||
| por24 | 445 | ABZ09841 | 65/75 | glutamine synthetase | ||
| por25 | 807 | WP_003888341 | 63/76 | Aminomethyltransferase | ||
| por26 | 352 | WP_010304927 | 69/79 | O-methyltransferase | ||
| por27 | 448 | WP_005445166 | 73/85 | Flavin-containing monooxygenase FMO |