Table 3. Tm (K), ΔH (kJ mol–1) and ΔG (kJ mol–1 at 300 K) values for melting and annealing of the five oligonucleotides, determined in 10 mM lithium phosphate pH 7.4, containing different concentrations of potassium chloride (mM).
| |
|
GGG-TTA |
|
GGG-H2 |
|
GGG-H1 |
|
GGG-Prop |
|
GGG-Oct |
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ΔH | Tm | ΔG | ΔH | Tm | ΔG | ΔH | Tm | ΔG | ΔH | Tm | ΔG | ΔH | Tm | ΔG | ||
| 40 |
Melt |
262 |
342/341 |
31.8 |
276 |
343/342 |
34.2 |
|
|
|
|
–/347 |
|
|
|
|
| |
Anneal |
273 |
341/340 |
32.5 |
295 |
343/342 |
36.6 |
|
|
|
|
–/344 |
|
|
|
|
| 10 |
Melt |
234 |
331/330 |
22.0 |
240 |
330/330 |
22.1 |
|
|
|
|
–/339 |
|
|
|
|
| |
Anneal |
248 |
331/330 |
23.1 |
236 |
330/328 |
21.2 |
|
|
|
|
–/337 |
|
|
|
|
| 5 |
Melt |
219 |
327/326 |
17.8 |
238 |
326/325 |
18.9 |
279 |
347/346 |
37.9 |
76.9 |
337/334 |
8.3 |
|
–/359 |
|
| |
Anneal |
227 |
327/326 |
18.5 |
210 |
324/323 |
15.6 |
287 |
346/345 |
38.1 |
81.1 |
337/336 |
8.9 |
|
–/361 |
|
| 2 |
Melt |
200 |
321/320 |
12.9 |
243 |
320/319 |
14.9 |
264 |
341/340 |
31.5 |
87.9 |
328/330 |
7.6 |
354 |
355/353 |
54.7 |
| |
Anneal |
193 |
320/319 |
12.1 |
168 |
315/314 |
7.7 |
285 |
340/339 |
33.4 |
88.6 |
329/328 |
7.9 |
|
–/355 |
|
| 1 |
Melt |
178 |
314/315 |
7.9 |
233 |
314/315 |
10.5 |
250 |
336/334 |
31.9 |
91.0 |
324/323 |
6.7 |
308 |
349/348 |
43.2 |
| |
Anneal |
167 |
314/314 |
7.2 |
146 |
308/309 |
3.7 |
271 |
335/335 |
28.5 |
92.4 |
325/326 |
7.1 |
306 |
348/348 |
42.4 |
| 10 mM Li |
Melt |
|
|
|
|
|
|
135 |
310/311 |
4.4 |
52.0 |
314/– |
2.4 |
167 |
328/325 |
14.1 |
| Anneal | 151 | 311/311 | 5.5 | 59.0 | 316/– | 3.0 | 197 | 328/326 | 17.0 | |||||||
The samples were heated and cooled at a rate of 0.2 K min–1. The first value for Tm was determined from van’t Hoff analysis of the melting profiles, while the second (in italics) was estimated from the maximum in the first derivative of the melting profile, using the LightCycler programme.