Figure 5.

H/R-mediated induction of Egr-1 in rat alveolar MPs (NR8383): effect of inhibitors of PKCβ and MAPKs. Rat MPs were incubated with LY379196 (200 nM), PD98059 (50 ∝M), SP600125 (20 ∝M), and SB203580 (10 ∝M), respectively, for 1 hour before being subjected to hypoxia for 30 minutes followed by reoxygenation for 15 minutes. Comparison is with H/R alone or normoxia (N). Real-time PCR analysis of total RNA from the above cells was performed. Data are presented as the fold induction of mRNA for Egr-1 normalized to β-actin. These experiments were repeated five times. *P < 0.001.