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. 2004 Jun 1;113(11):1596–1606. doi: 10.1172/JCI20741

Figure 4.

Figure 4

Fibrinogen γ390–396A does not support αMβ2-dependent adhesion of primary neutrophils. Primary human neutrophils (106 cells/ml) were transferred to either uncoated wells, wells coated with 10 ∝g/ml WT mouse fibrinogen (A), wells coated with 10 ∝g/ml fibrinogen γ390–396A (B), or wells coated with 10 ∝g/ml fibrinogen γØ5 (C). Shown are representative views of bound cells after 25 minutes. (D) Quantitative comparison of neutrophil adhesion to WT, fibrinogen γ390–396A, and fibrinogen γØ5. The specificity of neutrophil engagement was established by preincubating neutrophils with 20 ∝g/ml of the rat anti-mouse αM I-domain Ab, M1/70, or control rat anti-mouse IgG for 15 minutes at room temperature before addition to the fibrinogen-coated wells. The data shown are means ± SD. HPF, high power field; Con, control.