Figure 1.

Transcriptional upregulation of cytokine genes by NKT cells stimulated with glycolipids in vivo. B6 mice were injected intraperitoneally with αGC or OCH (100 ∝g/kg), and liver NKT cells were isolated at the indicated time point. Total RNA was extracted and analyzed for cytokine mRNA by quantitative RT-PCR as described in Methods. Data are presented as “fold induction” of cytokine mRNA after glycolipid treatment. The amount of mRNA in NKT cells derived from untreated animals was defined as 1.