Table 2. Intracellular S-nitrosothiol formation after incubation of RAW 264.7 cells with spermine NONOate.

	S-nitrosothiol content, pmol/mg of protein
HBSS	46.2 ± 2.5
Medium	29.4 ± 2.3
HBSS + l-cystine	51.2 ± 3.2
HBSS + l-cysteine	487.8 ± 56.0

RAW 264.7 cells were treated with spermine NONOate (500 μM) for 60 min in HBSS, FBS-free medium, or HBSS supplemented with l-cystine or l-cysteine (200 μM). The cells were washed and lysed. The S-nitrosothiol content in the cell lysate was measured by chemiluminescence. Data represent mean ± SEM (n = 3).