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Effect of viscosity on kcat/KM of peptide bond formation on the ribosome. kcat/KM was measured in the absence [(kcat/KM)°] or presence of increasing trehalose concentrations. η° and η are viscosities of the reaction solutions in the absence and presence of trehalose, respectively. The dashed line (slope = 1) shows the behavior expected if the rate of reaction were limited by binding of the substrate (Pm).
