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Energy requirement for OEP80tr import in vitro. Translation products including the radiolabeled proteins indicated at left (tl) were incubated without (–) or with (+) apyrase at room temperature for 15 min in the light. The resultant samples were incubated with isolated chloroplasts without (–) or with (+) 3 mM MgATP for 30 min at room temperature in the dark. Chloroplasts were re-isolated, fractionated and examined as described in Figure 4A.
