Table 1.
Antibodies used for Immunohistochemistry.
| Primary Antibody | Immunogen | Host | Code/clone | Dilution | Manufacturer | Tissue processing |
|---|---|---|---|---|---|---|
| Iba1 | C-terminus of Iba1′ (N′-PTGPPAKKAISELP-C′) | Rabbit | 019–19741, Lot #CDQ5232 | 1:2000 | Wako Pure Chemical Industries, Neuss, Germany | LM, CM, EM |
| CD11b | Rat peritoneal macrophages | Mouse | MCA275G | 1:100 | Serotec, Oxford, UK | CM |
| GFAP | Cow spinal cord GFAP | Rabbit | Z0334 | 1:2000 | Dako, Glostrup, Denmark | LM, EM |
| GFAP | Porcine spinal cord GFAP | Mouse | MAB360 | 1:2000 | Millipore, Billerica, MA, USA | CM |
| NeuN | Purified cell nuclei from mouse brain | Mouse | MAB337, Lot # LV1825845 | 1:200 | Millipore, Billerica, MA, USA | CM |
| NT-3 | Amino-terminal of mouse NT-3 (H-YAEHKSHRGEY-NH2) | Rabbit | AB1532SP | 1:100 | Millipore, Billerica, MA, USA | LM,CM |
LM, light microscopy; CM, confocal microscopy; EM, electron microscopy.