Figure 1.
In vitro recruitment of dendritic cells and macrophages by GM-CSF-loaded hydrogels. (A) mPEG–PLGA copolymer formulations are an injectable solution at 4 °C, but turn to gel after incubation at 37 °C for 5 min. (B) The release profiles of FITC-labeled GM-CSF from hydrogel formulations (15, 20, and 25 wt %; n = 3). (C) In vitro chemotaxis using a transwell migration assay. Chemotaxis of BMDCs and BMDMs in response to hydrogels encapsulating various doses of GM-CSF was determined by counting the number of BMDCs and BMDMs migrating to the lower compartment of transwell (n = 3).