Fig. 5.

Biochemical characterization of ovine endometrial galectin-15. (A) Carbohydrate-binding analysis of galectin-15 from the uterine lumen of day-16 pregnant ewes by using lactose-agarose and competition with lactose, mannose, or fucose. (B) Immunological identification of galectin-15 from the uterine lumen of day-16 pregnant ewes (study 1) and as the previously uncharacterized 14K protein. (C) Immunological identification of galectin-15 from uterine milk (study 3) as the 14K protein. The anti-oTP-1/oIFN-τ antibody crossreacts with the 14K protein (22), which is ovine endometrial galectin-15.