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. 2014 May 13;210(9):1487–1498. doi: 10.1093/infdis/jiu287

Figure 4.

Figure 4.

Glycosylated surface structures of Campylobacter jejuni flagella bind to Siglec-10 receptor. A, 11168H WT, flaA, Cj1316, and waaF mutants were assessed for binding to CHO cells overexpressing Siglec-10 receptor at 4°C. Histograms are representative of 3 independent experiments. Control cells alone (dotted line), bacteria/CHO cell co-cultures (solid gray). B, Binding of 11168H WT and Cj1316 mutant to CHO cells is expressed as a bacterial binding index (% cells with bacteria bound × geometric mean fluorescence intensity of the bacteria-positive cells). Bars depict mean values ± SEM from 5 independent experiments. C, The binding capacity of Siglec-10-Fc to purified flagella from WT 11168H, WT 81-176 and 11168H Cj1316 mutant was assessed by ELISA. Bars depict mean values ± SEM from 3 independent experiments performed in duplicate. Friedman statistical analysis was performed on data sets. *P < .05; **P < .01; ***P < .001. Abbreviations: CHO, Chinese hamster ovary; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; MOI, multiplicity of infection; SEM, standard error of the mean; WT, wild-type.