Fig. 4.

The requirement of IKKα/β for IFNγ-stimulated IP10 gene expression is independent of NF-κB activation. (a) The response of IP10 to IFNγ does not require p65. WT or p65NFκB-null cells were treated with IL-1, TNF, or IFNγ for 6 h or were untreated, and total RNA was analyzed by the Northern procedure. (b) The response of IP10 to IFNγ is not inhibited by the IκBα super repressor. WT cells or cells expressing the IκBαSR were treated with IFNβ, IFNγ, IL-1, or TNF for 6 h or were untreated, and total RNA was analyzed by the Northern procedure.