Table 1.

Results of assay validation parameters obtained by applying the proposed methods.

Parameter	Ezetimibe				Atorvastatin calcium
		IS
	D1	224.6 nm	238.6 nm	TLC-densitometry	D1	D2	TLC-densitometry
Concentration range	10.00–30.00 (μg mL−1)	10.00–35.00 (μg mL−1)	5.00–30.00 (μg mL−1)	0.10–0.60 (μg band−1)	10.00–35.00 (μg mL−1)	10.00–35.00 (μg mL−1)	0.10–0.60 (μg band−1)
Linearity
Slope	0.0170	0.0365	0.0430	4656.6857	0.0060	0.0349	5165.4857
Intercept	0.0219	0.0137	0.0316	−190.7733	−0.0012	−0.0047	76.7133
Correlation coefficient (r)	0.9995	0.9995	0.9997	0.9998	0.9998	0.9994	0.9999
Standard error of the slope	0.0003	0.0006	0.0005	42.5386	0.00005	0.0006	33.3275
Confidence limit of the slope	0.0170 ± 0.0008	0.0365 ± 0.0016	0.0430 ± 0.0014	4656.6857 ± 118.1060	0.0060 ± 0.0002	0.0349 ± 0.0017	5165.4857 ± 92.5321
Standard error of the intercept	0.0057	0.0140	0.0095	16.5664	0.0013	0.0144	12.9792
Confidence limit of the intercept	0.0219 ± 0.0158	0.0137 ± 0.0389	0.0316 ± 0.0265	−190.7733 ± 45.9957	−0.0012 ± 0.0036	−0.0047 ± 0.0399	76.7133 ± 36.0361
Accuracy (Mean ± S.D.)	100.23 ± 1.62	99.75 ± 1.43	99.80 ± 0.95	99.77 ± 1.30	99.58 ± 0.84	99.72 ± 1.36	99.86 ± 0.97
Precision (RSD %)
Repeatabiltya	0.96	0.45	0.48	1.00	1.24	1.14	0.97
Intermediate precisionb	1.16	1.31	1.10	1.40	1.32	1.34	1.29
Specificity	99.66 ± 1.03	100.89 ± 0.89	100.47 ± 0.81	100.25 ± 0.82	99.39 ± 0.81	100.47 ± 1.06	100.49 ± 0.78
Limit of detection (LOD)c	2.70 μg mL−1	3.10 μg mL−1	1.79 μg mL−1	0.03 μg band−1	1.75 μg mL−1	3.33 μg mL−1	0.02 μg band−1
Limit of quantitation (LOQ)c	8.19 μg mL−1	9.41 μg mL−1	5.43 μg mL−1	0.09 μg band−1	5.31 μg mL−1	10.09 μg mL−1	0.06 μg band−1

LOD = (SD of the response/slope) × 3.3; LOQ = (SD of the response/slope) × 10.

^aThe intraday (n = 3), average of three concentrations repeated three times within day.

^bThe interday (n = 3), average of three different concentrations repeated three times in three successive days.

^cLimits of detection and quantitation are determined via calculations.