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. 2014 May 5;20(19-20):2656–2665. doi: 10.1089/ten.tea.2013.0570

FIG. 4.

FIG. 4.

(A) Morphology of the HDMEC cultured on PC collagen constructs after 3 weeks in culture. Endothelial cells adhere to the PC collagen and retain characteristic cobblestone morphology with expression of endothelial cell marker PECAM-1 between cell–cell contacts (nuclei counterstained with 4′,6-diamidino-2-phenylindole [DAPI]). (B) SEM imaging of HDMEC on the PC constructs showed cells (arrows) adherent to the fibrillar collagen surface. (C) Histological examination of the PC constructs revealed a monolayer of adherent endothelial cells. (D, E) HDMEC stained positively for expression of basement membrane proteins laminin (D) and collagen type IV (E). Arrows indicate endothelial cells on the images.