Figure 1.

Scheme of the MiSeq sequencing library in this study. The 150-bp paired reads (black arrow) represent the junction sequence of the retrotransposon (Rtsp-1 or LIb) (red box) and the insertion site sequence from the AP3 adapter (purple box). The TruSeq adapters used in this study were indexed with short 6–8 bp sequences to facilitate multiplexing in a single sequencing run. The P5 and P7 sequences (blue box) allowed the final products to hybridize to the Illumina flow cell. The Rd1 and Rd2 sequences (green box) served as sequencing primers.