Table 3.
Summary of blastn results against genome sequences
| Mapped sequences | Hits against draft genome | Hits against barley genome | Hits on the same homoeologous chromosomes |
|---|---|---|---|
| A genome | 3,918 (87.8%) | 2,856 (64.0%) | 1,658 (37.2%) |
| 1A | 464 (84.5%) | 379 (69.0%) | 265 (48.3%) |
| 2A | 488 (91.6%) | 338 (63.4%) | 238 (44.7%) |
| 3A | 458 (87.2%) | 333 (63.4%) | 238 (45.3%) |
| 4A | 851 (85.2%) | 608 (60.9%) | 85 (8.5%) |
| 5A | 416 (91.4%) | 315 (69.2%) | 184 (40.4%) |
| 6A | 555 (85.6%) | 409 (63.1%) | 293 (45.2%) |
| 7A | 686 (91.1%) | 474 (62.9%) | 355 (47.1%) |
| B genome | – | 3,972 (62.4%) | 1,772 (27.8%) |
| 1B | – | 629 (63.4%) | 279 (28.1%) |
| 2B | – | 793 (64.4%) | 389 (31.6%) |
| 3B | – | 568 (61.3%) | 249 (26.9%) |
| 4B | – | 290 (58.7%) | 150 (30.4%) |
| 5B | – | 588 (60.9%) | 268 (27.8%) |
| 6B | – | 573 (61.9%) | 207 (22.4%) |
| 7B | – | 531 (63.8%) | 230 (27.6%) |
| D genome | 1,319 (59.2%) | 1,006 (45.2%) | 923 (41.4%) |
| 1D | 216 (64.7%) | 150 (44.9%) | 110 (32.9%) |
| 2D | 514 (61.6%) | 436 (52.3%) | 417 (50.0%) |
| 3D | 230 (59.3%) | 181 (46.6%) | 176 (45.4%) |
| 4D | 30 (41.7%) | 23 (31.9%) | 23 (31.9%) |
| 5D | 131 (52.6%) | 90 (36.1%) | 89 (35.7%) |
| 6D | 90 (50.3%) | 68 (38.0%) | 64 (35.8%) |
| 7D | 108 (62.8%) | 58 (33.7%) | 44 (25.6%) |
| Total | 5,237 (78.3%) | 7,834 (60.0%) | 4,353 (33.3%) |
Sequences mapped on A- and D-genomes were first blast searched against T. urartu and Ae. tauschii genome sequences and then the obtained scaffolds were assigned to the barley physical map based on their genes. B-genome sequences were directly aligned to the barley genome.