| # of vials | Total Seeding Volume (ml) | Flask |
| 1 | 5 | T12.5 |
| 2 | 10 | T25 |
| 3 | 15 | T75 |
| 4 | 20 | T75 |
| 5 | 25 | T75 |
| 6 | 30 | T175 |
| 7 | 35 | T175 |
| 8 | 40 | T175 |
| 8 + | -- | Combination of Flasks |
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