Flask	Volume of total media	Pre-Chop	→	Post-Chop
1 T12.5	5 ml	1 T12.5	→	1 T25
1 T25	10 ml	1 T25	→	1 T75
1 T75	20 ml	1 T75	→	1 T175
1 T175	40 ml	1 T175	→	2 T175s
2 T175s	80 ml	2 T175s *	→	4 T175s
* 2 T175s is the maximum number of flasks that can be chopped at a  time.  Chop in sets of 2 T175s and refer to step 7.