Column A		Column B	Column C	Column D	Column E	Column F
Pre-Chop Flask Size	→	Post-Chop Flask(s) Size	Suggested volume of MM to transfer to new flask(s) pre-chop	Suggested volume of CM to transfer to new flask(s) pre-chop	Suggested volume of spheres/media to transfer into new flask(s) post-chop	Final Volume Seeded/Flask
T12.5	→	T25	5 ml	0 ml	5 ml	10 ml
T25	→	T75	10 ml	0 ml	10 ml	20 ml
T75	→	T175	20 ml	10 ml	10 ml	40 ml
1 T175	→	2 T175s	20 ml per flask	15 ml per flask	5 ml per flask	40 ml
2 T75s	→	4 T175s	20 ml per flask	17.5 ml per flask	2.5 ml per flask	40 ml