FIG. 2.

Multiphoton microscopy and hematoxylin and eosin (H&E) staining of human umbilical vein endothelial cells (HUVEC) and HUVEC/human aortic smooth muscle cells (HASMC)-seeded microchannels after 7 days of static culture. Coronal multiphoton micrograph after 7 days of culture in a microchannel seeded with HUVEC (a) and three-dimensional (3D) reconstruction (b) of optical stack with adherent autoflourescent cells along microchannel luminal wall. (c) Coronal multiphoton micrograph of HUVEC/HASMC-seeded microchannel and 3D reconstruction (d) after 7 days of culture depicting a confluent lining of cells along the luminal surface. H&E-stained HASMC/HUVEC-seeded microchannel (e, f) and HUVEC-only-seeded microchannel (g, h) after 7 days. Note the areas of HUVEC delamination within HUVEC-only-seeded microchannels (oval). Color images available online at www.liebertpub.com/tea