FIG. 1.

Polyacrylamide gel fabrication and conjugation. (A) Polyacrylamide gel structure. (B) Hydrazine treatment of the gel surface yields hydrazide groups that react with the activated proteins to covalently attach the protein to the gel surface. (C) Left: Fluorescence measurements made with Alexa-546 fibrinogen to confirm protein immobilization. Right: Fluorescence image of non-hydrazine treated (top) versus hydrazine treated (bottom). Error bars represent standard deviation from at least three replicates (n=3). Color images available online at www.liebertpub.com/tea