FIG. 4.
MSC patterning to restrict cell area under the fibronectin condition. (A) Fibronectin was patterned on the surface of the gels via soft lithography with a polydimethylsiloxane stamp into 3000 μm2 islands. (B) Immunofluorescence image of cells patterned on the surface of a 10-kPa gel (magnified view in inset; green—actin). (C) Effect of restricting cell area on HMVEC tubulogenesis. Error bars represent standard deviation from three replicates (n=3). †p<0.01. Color images available online at www.liebertpub.com/tea