Fig. 1.

(A) Size exclusion chromatography showing XET activity between high-molecular-weight xyloglucan and 2-amimopyridyl xyloglucan oligosaccharide (XXXG-PA). A mixture of tamarind xyloglucan (donor substrate) and 2-amimopyridyl xyloglucan oligosaccharide XXXG-PA (acceptor substrate) was incubated for 1 h at 30 °C with native or denatured recombinant OsXTH11 protein (as indicated in the key). After the reaction, the reaction mixture was separated by size exclusion chromatography, and the elution profile of the high-molecular-weight fluorescently labelled product was measured with a fluorescence detector. PAD, pulsed amperometric detector. (B) pH dependency of OsXTH11-mediated XET activity.