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The prp3 vti13 double mutant was much less sensitive to mannitol in the growth medium when compared with the single mutants and appeared similar in root hair phenotype to wild-type seedlings under both growth conditions. (A, E) Wild type (B, F), prp3, (C, G) vti13 and (D, H) prp3 vti13 double mutants were grown on 1× MS medium (A–D) and 1× MS medium including 200 mm mannitol (E–H). Scale bars: (A–H) = 50 μm.
