Fig. 5.

VTI13-labelled compartments are sensitive to BFA. Scanning laser confocal micrographs of 35S:GFP–VTI13 (green) seedlings treated with FM4-64 (red) and (A) DMSO, (B) 10 μm BFA or (C) 50 μm BFA for 10 min only exhibited sensitivity at higher concentrations of BFA. Still frames from each treatment were taken from live images of treated root hairs (Supplementary Data Videos S4–S6). Arrows point to VTI13-labelled compartments whose mobility was not altered by treatment with (A) DMSO or (B) 10 μm BFA, but became swollen and less mobile when treated with (C) 50 μm BFA. Scale bars: (A–C) = 5 μm.