Skip to main content
. 2014 Oct 13;9(10):e107388. doi: 10.1371/journal.pone.0107388

Figure 3. Intracellular calcium [Ca2+]i response in GC-GHRHR cells mediated by GPR41 and 43 receptors.

Figure 3

(A) GPR41 and GPR43 expression was detected by RT-PCR in GC-GHRHR cells. The expected sizes of the amplified DNA bands were 439 and 508 bp for GPR43 and GPR41, respectively. (B) Measurements of the [Ca2+]i in GC-GHRHR cells, transiently transfected with wt-hGH and treated with butyrate, were assessed after stimulation with 10 nM GHRH and/or 5 mM butyrate. Addition of the stimulus is indicated with an arrow. One representative experiment of at least 5 independent experiments is shown (*, p<0.05, **, p<0.01, ***p<0.001).