| Tube Number | Volume (μl) | Dye stock (mM CFSE) | Final concentration (μM) |
| 37 - 42 | 0 | 0 | 0 |
| 31 - 36 | 7 | 0.022 | 0.139 |
| 25 - 30 | 7 | 0.078 | 0.492 |
| 19 - 24 | 7 | 0.23 | 1.45 |
| 13 - 18 | 7 | 0.82 | 5.17 |
| 7 - 12 | 7 | 2.9 | 18.3 |
| 1 - 6 | 7 | 10 | 63.1 |
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