| Tube Number | Volume (μl) | Dye stock (mM CPD) | Final concentration (μM) |
| A | 0 | 0 | 0 |
| B | 4 | 0.053 | 0.106 |
| C | 6.3 | 0.197 | 0.62 |
| D | 7.5 | 0.73 | 2.74 |
| E | 7.3 | 2.7 | 9.86 |
| F | 7.7 | 10 | 38.5 |
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