Table 3. Human γ-globin gene expression in transgenic mice carrying the construct μLCR (-159h)Aγ (TATAR) or μLCR(-157g)Aγ(TATAh).
| Human γ, % of murine α-mRNA per copy
|
|||||
|---|---|---|---|---|---|
| Embryonic erythropoiesis
|
Definitive erythropoiesis
|
||||
| Line | Copy no. | Day 12 blood | Day 12 yolk sac | Day 12 fetal liver | Adult blood |
| μLCR(-159h)Aγ(TATAR) | |||||
| P | 45 | 21.2 ± 4.0 | |||
| Q | 11 | 22.0 ± 1.3 | 44.8 ± 12.6 | 31.6 ± 5.9 | 15.5 ± 2.1 |
| R | 8 | 21.1 ± 2.2 | |||
| S | 6 | 21.8 ± 4.8 | 34.9 ± 3.6 | 17.0 ± 1.1 | 13.4 ± 1.7 |
| T | 25 | 18.5 ± 4.7 | 50.9 ± 13.1 | 12.7 ± 1.4 | 18.0 ± 6.5 |
| Mean | 20.8 ± 2.0 | 43.5 ± 8.1 | 20.4 ± 9.9 | 17.8 ± 3.4 | |
| μLCR(-157g)Aγ (TATAh) | |||||
| U | 28 | 16.4 ± 2.8 | 32.3 ± 5.4 | 13.8 ± 0.5 | 5.7 ± 0.7 |
| V | 9 | 27.9 ± 3.9 | 37.0 ± 6.7 | 18.5 ± 5.1 | 6.9 ± 1.6 |
| W | 2 | 16.2 | 17.3 | 10.4 | 4.7 ± 2.0 |
| X | 12 | 24.0 ± 3.3 | 38.0 ± 3.3 | 16.9 ± 3.7 | 5.9 ± 0.7 |
| Mean | 21.1 ± 6.0 | 31.2 ± 9.6 | 14.9 ± 3.6 | 5.8 ± 0.9 | |
| μLCR(-159h)Aγ | |||||
| Mean | 9.0 ± 5.5 | 26.6 ± 8.8 | 14.0 ± 4.9 | 14.3 ± 3.3 | |
See Table 1 legend and footnote.