Fig. 1.

CD40 ligation, but not hBAFF or LPS treatment, of primary B cells activates both type 1 and type 2 NF-κB activity. (A) p100 processing to p52. WT B cells were cultured in medium alone (MC) or in the presence of hBAFF, anti-CD40 mAb, or LPS for 24 h. Whole-cell lysates were Western-blotted for p52 and actin (loading control). (B) p65 and p52 nuclear translocation. WT B cells were cultured with hBAFF, anti-CD40 mAb, or LPS for the indicated times. Nuclear extracts were Western-blotted for p65, p52, and upstream stimulatory factor 2 (USF2; loading control). Data are representative of three experiments.