Skip to main content
. 2014 Oct 13;9(10):e109655. doi: 10.1371/journal.pone.0109655

Figure 5. Luteolin exhibits selective cytotoxicity towards tumorigenic cells and induces apoptosis.

Figure 5

(A), The effects of luteolin on cell viability of tumorigenic (HeLa and U2OS) and non-tumorigenic (BEAS-2B and HEK293A) cell lines. Cells were treated with indicated concentration of luteolin for 24 hours. Afterward, cell viability was analyzed by the MTT assay. (B), Time-dependent effects of luteolin on HeLa cell viability. HeLa cells were treated with indicated luteolin 10 µM for indicated times. Cell viability was assessed by the MTT assay. Error bars represents means ±SEM (n = 10). Symbols (***) represent p value <0.0001. (C), The effects of flavonoids on A549 cell viability were compared. Error bars represent means ±SEM (n = 10). Symbols (***) represent p value <0.0001. The P-values in (A), (B) and (C) were calculated using Student's t-test. (D), Proapoptotic effect of luteolin on HeLa cells. HeLa cells treated with indicated concentrations of luteolin were double stained with PI/Annexin-V allophycocyanin. Apoptosis analysis was performed by flow cytometry.