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. 2014 Oct 13;9(10):e109590. doi: 10.1371/journal.pone.0109590

Figure 6. Detection of S. invicta sNPF receptor in cell membranes prepared from the SisNPFR-C6E8 cell line by western blot analysis.

Figure 6

Numbers in the left indicate the marker’s protein mass (kDa). Lane 1, the membrane fraction of the fire ant mated queen ovary (Ov) as a positive control; lane 2, the membrane fraction of SisNPFR-C6E8 (C6E8) and lane 3, the membrane fraction of the vector-only (VO) (pcDNA3.1(-)) transfected CHO-K1 cells probed with the specific anti-sNPF receptor anti-peptide antibody [44]. Membrane protein (50 µg) was loaded in each lane. The antibody detects the 55 kDa receptor protein in lanes 1 and 2, as expected.