Table 1. Amino acid sequences of the ligands tested on the SisNPFR-C6E8 cell line and S. invicta peptides EC₅₀ values.

Peptide ligands	Amino acid sequences (a = amidated at C-terminus)	Activity (EC50)	% identity to S. invictasNPF1
S. invicta sNPF1	SLRSALAAGHLRYa	3.2 nM	100
S. invicta sNPF2	SALAAGHLRYa	8.6 nM	100
Analogous sNP(F)	SLRSALAAGHLRFa	not active1 µM	92.3
Drome sNPF1	AQRSPSLRLRFa	not active1 µM	50
Drome sNPF2	WFGDVNQKPIRSPSLRLRFa	not active1 µM	40
Drome sNPF212–19	SPSLRLRFa*	not active1 µM	42.9
Mouse PYY	AKPEAPGEDASPEELSRYYASLRHYLNLVTRQRYa	not active1 µM	30.8
Apime NPY	EPEPMARPTRPEIFTSPEELRRYIDHVSDYYLLSGKARYa	not active1 µM	38.5

S. invicta (Solenopsis invicta), Analogous sNP(F) (Analog of S. invicta sNPF1), Drome (Drosophila melanogaster), Apime (Apis mellifera).

*Sequence identical to Apime sNPF.

All other peptides tested were not active at 1 µM.