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. 2004 May 12;101(21):8132–8137. doi: 10.1073/pnas.0402088101

Fig. 3.

Fig. 3.

Hypoxia-induced syndecan-4 and α5-integrin expression and adhesion to fibronectin of colon cancer cells cultured under hypoxic conditions. (A) Signals of syndecan-4 and α5-integrin in the DNA microarray (white arrows). Cy3-dUTP (normoxia: red) or Cy5-dUTP (hypoxia, 1% O2 for 24 h: green) was incorporated into cDNA prepared from a human colon cancer cell line SW480. (B) SW480 cells were cultured under 1% O2 for 7 days and subsequently under 20% O2 for an additional 14 days and analyzed for syndecan-4 and α5-integrin expression by flow cytometry. (C) Hypoxia-induced enhancement of SW480 cell adhesion to immobilized fibronectin. Filled bars, adhesion to immobilized fibronectin; open bars, adhesion to BSA (negative control).